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Hong-Gyun Lee, PhD

Job Title

Postdoctoral Fellow

Academic Rank

Fellow or Postdoc




Hong-Gyun Lee

Principal Investigator

Francisco J. Quintana



Droplet-based forward genetic screening of astrocyte–microglia cross-talk

Scientific Abstract

Cell–cell interactions in the central nervous system play important roles in neurologic diseases. However, little is known about the specific molecular pathways involved, and methods for their systematic identification are limited. Here, we developed a forward genetic screening platform that combines CRISPR-Cas9 perturbations, cell coculture in picoliter droplets, and microfluidic-based fluorescence-activated droplet sorting to identify mechanisms of cell–cell communication. We used SPEAC-seq (systematic perturbation of encapsulated associated cells followed by sequencing), in combination with in vivo genetic perturbations, to identify microglia-produced amphiregulin as a suppressor of disease-promoting astrocyte responses in multiple sclerosis preclinical models and clinical samples. Thus, SPEAC-seq enables the high-throughput systematic identification of cell–cell communication mechanism.

Lay Abstract

We developed a novel method to identify molecular mechanisms of cell–cell communication that controls multiple sclerosis (MS).

Clinical Implications

We discovered a novel cross-talk between astrocyte-microglia that can modulate the progression of multiple sclerosis (MS).