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Ming Tao, MD
Pronouns
Job Title
Investigator
Academic Rank
Instructor
Department
Surgery
Authors
Ming Tao, Peter Kip, Thijs Sluiter, Michael MacArthur, Sarah Mitchell, Margreet R de Vries, Masanori Aikawa, and C. Keith Ozaki
Principal Investigator
C. Keith Ozaki MD
Research Category: Cardiovascular, Diabetes, and Metabolic Disorders
Tags
Objective: Obesity is an independent risk factor for cardiovascular disease and revascularization failure. Dietary methionine restriction (MetR), as a novel interventional modality, improves perivascular adipose tissue (PVAT) metabolic fitness and reduces surgical stress in preclinical models. We hypothesized that one-week of pre-operative MetR attenuates vein graft disease.
Methods: Male 12-week-old diet induced obesity (DIO) mice were fed a control diet (60% fat) for 3 weeks, followed by 1 week of MetR (60% fat/0.05% methionine) prior to surgery. 20 mice (n=10) underwent unilateral carotid interposition allografting via a cuff technique (donor mice from the same diet/genetic background, vein cava PVAT attached). All mice were returned to the control diet post-surgery. The grafts were harvested at day 28 for analyses.
Results: MetR led to approximately 17.9% body weight loss. MetR significantly reduced intima area, intima/(media+adventitia) area ratios and improved the remodeling index(p<0.05). Furthermore, short-term MetR significantly increased macrophage M2/M1 ratios in the graft wall(p<0.05).
Conclusions: MetR intervention prior to surgery significantly reduces intimal hyperplasia in DIO mice and is associated with increased M2 macrophage populations. It stands as a simple, translatable strategy for increasing host resistance to surgical trauma and improving vein graft durability.
Objective: Obesity is an independent risk factor for cardiovascular disease and revascularization failure. Dietary methionine restriction (MetR), as a novel interventional modality, improves perivascular adipose tissue (PVAT) metabolic fitness and reduces surgical stress in preclinical models. We hypothesized that one-week of pre-operative MetR attenuates vein graft disease.
Methods: Male 12-week-old diet induced obesity (DIO) mice were fed a control diet (60% fat) for 3 weeks, followed by 1 week of MetR (60% fat/0.05% methionine) prior to surgery. 20 mice (n=10) underwent unilateral carotid interposition allografting via a cuff technique (donor mice from the same diet/genetic background, vein cava PVAT attached). All mice were returned to the control diet post-surgery. The grafts were harvested at day 28 for analyses.
Results: MetR led to approximately 17.9% body weight loss. MetR significantly reduced intima area, intima/(media+adventitia) area ratios and improved the remodeling index(p<0.05). Furthermore, short-term MetR significantly increased macrophage M2/M1 ratios in the graft wall(p<0.05).
Conclusions: MetR intervention prior to surgery significantly reduces intimal hyperplasia in DIO mice and is associated with increased M2 macrophage populations. It stands as a simple, translatable strategy for increasing host resistance to surgical trauma and improving vein graft durability.
