Efficacy of ACELL Cytal® Wound Matrix with and without Micromatrix®

Qinxin Liu, MD
Department of Medicine
Division of Endocrinology, Diabetes, and Hypertension
Poster Overview

Objective: Biomaterials in various forms are extensively used in wound healing. In this study we assessed the efficacy of a porcine-derived powder matrix alone or in combination with a sheet scaffold in diabetic wound healing.

Methods: 1×1 cm2 full-thickness dorsal skin wounds were excised on a total of 60 diabetic mice. Mice were either untreated (Blank, n=15), or treated with a powder matrix (MicroMatrix™; Mx, n=15), scaffold sheet alone (Cytal® Wound Matrix Layer 1; C1, n=15), or scaffold sheet over powder matrix (C1+Mx, n=15). Wounds were photographed on Day 0, 5, 10, 14 and 21 post surgery. Ten animals from each group were sacrificed on day 10 and wound tissue was harvested for further analysis.

Results: No difference in the wound healing rate was noted between all groups. The C1 and C1+Mx groups showed significantly higher wound bed growth compared to the Blank group (p<0.001). The C1+Mx group showed significantly higher collagen deposition than the Blank (p<0.01) and C1 (p<0.05) groups, which promoted wound healing. Cellular proliferation was significantly higher in the C1 and C1+Mx groups than the Blank or Mx groups.

Conclusions: Without impacting the wound healing rate, combination of powder and sheet matrix resulted in improved wound healing.

Scientific Abstract

Objective: Decellularized scaffolds in various forms are extensively used in wound healing. In this study we assessed the efficacy of a porcine-derived urinary bladder powder matrix alone or in combination with a non-crosslinked sheet scaffold in diabetic wound healing.

Methods: 1×1 cm2 full-thickness dorsal skin wounds were excised on a total of 60 db/db mice. Mice were either untreated (Blank, n=15), or treated with a powder matrix (MicroMatrix™; Mx, n=15), scaffold sheet alone (Cytal® Wound Matrix Layer 1; C1, n=15), or scaffold sheet over powder matrix (C1+Mx, n=15). Wounds were photographed on Day 0, 5, 10, 14 and 21 post surgery. Ten animals from each group were sacrificed on day 10 and wound tissue was harvested for histological analysis.

Results: No difference in the wound healing rate was noted between all groups. The C1 and C1+Mx groups showed significantly higher wound bed thickness compared to the Blank group (p<0.001). The C1+Mx group showed significantly higher collagen deposition than the Blank (p<0.01) and C1 (p<0.05) groups. Cellular proliferation was significantly higher in the C1 and C1+Mx groups than the Blank or Mx groups.

Conclusions: Without impacting the wound healing rate, combination of powder and sheet matrix resulted in improved wound healing.

Clinical Implications
Refractory wounds are a great challenge for diabetic patients. Biomaterials are widely used for promoting wound healing. In this study we tested different biomaterials in different combinations for their effect on diabetic wound healing and achieved promising results.
Research Areas
Authors
Qinxin Liu, M.D., Adriana Panayi, M.D., Shimpo Aoki, M.D., Ryoko Hamaguchi, B.S., Mehran Kervar, M.D., Mengfan Wu, M.D., Chenhao Ma, M.D.
Principal Investigator
Dennis P. Orgill

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