Here, we propose to develop an autonomous in-situ bioprinting system, which will incorporate a 6-degree-of-freedom robot arm and an artificially intelligent (AI)-based image recognition algorithm and vision guidance system. This integrated system will allow for rapid collection of multiple views of the targeted defects from different angles, while the computer vision-based reconstruction system will dynamically create 3D constructs of the volumetric defect sites. It enables adaptive filling the defects customized to each patient’s defect geometry. In this research, as targeting to treat a diabetic wound healing, a microgel based bioink is introduced, which is mixed with a plant protein-based oxygenating microparticles and mesenchymal stem cells in order to boost angiogenesis and immune response at initial wound healing stage. We not only demonstrate the practicality for targeting and treating diabetic wounds using our system, highlighting its adaptability to enable customized care for rapid emergency treatment of trauma patients, but also verifying the efficiency of the combination of the oxygenated ink and the stem cells in wound repair.
Here, we propose a newly designed in situ bioprinting system that uses a robotic arm with six degrees of freedom and artificial intelligence for image recognition. This advanced system will swiftly capture multiple views of specific defects from various angles. Then, it will use computer vision to construct 3D models of these defect areas in real-time. This technology allows us to tailor the filling of these defects to match each patient’s unique geometry without the need for pre-set instructions or complex setup processes.
In addition, in our research, we focus on treating diabetic wounds. We have developed a special bioink made of micro-sized particle-based gels, which is combined with oxygenating microparticles derived from plant proteins and mesenchymal stem cells. This combination enhances blood vessel formation and the body’s immune response during the initial stages of wound healing. We not only show how effective our system is for targeting and treating diabetic wounds, demonstrating its adaptability for providing customized care in emergency situations, but also confirm the benefits of using oxygenated ink and stem cells for wound repair.